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KMID : 0939920020340010052
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대한암학회지
2002 Volume.34 No. 1 p.52 ~ p.57
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Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse
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Gi Yeong Huh/Kyung Seung Oh
Ki Uk Kim*/Na Hee Park†/Su Yeong Seo†/Sun Seob Choi‡/Gi Yeong Huh§
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Abstract
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Purpose: To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice.
Materials and methods: We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRalpha, CXCR4 and IL-4Ralpha.
Results: Expression of IL-4Ralpha, in implanted tumornodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Ralpha. The immunoreactivity of IL-4Ralpha, and other surface receptors such as CD71, IGF-IRalpha and EGFR, was prominent in tumor nodules associated with tumor necrosis.
Conclusion: IL-4Ralpha would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression.
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KEYWORD
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Glioblastoma, Interleukin-4 receptor, SCID mouse,
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